Molecular dynamics of targeting CD38 in multiple myeloma

Multiple functions of CD38 need exploring to expand clinical application anti-CD38 antibodies in multiple myeloma (MM). We investigated membrane dynamics MM cells and subsequent events when is targeted by therapeutic antibodies. Human (BF01) were co-cultured vitro with antibody (or control immunoglobulin G) analysed using gene expression profiling. Microvesicles from antibody-exposed for differential microRNA (miRNA) expression, phenotypic characterisation. Exposure BF01 resulted redistribution, upregulation metabolism-related genes downregulation involved cell cycle processes. derived showed increased CD73 CD39 presence programmed death-ligand 1 significant up-/down-modulation miRNAs. accumulated around Fc receptor-positive (FcR+) cells. Upon internalisation, natural killer displayed significantly related activation immune response, the cycle. Cells may use microvesicles transmit signals distally as part a survival strategy. are equipped on their surface enzymatic machinery leading production tolerogenic adenosine. Further, they internalised FcR+ functional modifications. These observations have relevance improving through targeting this mechanism its sequelae.